Substituted sulfonylacetamido cephalosporins

ABSTRACT

Cephalosporin compounds having various substituted sulfonylacetamido groups at position 7 have been prepared and have antibacterial activity.

United States Patent [191 De Marinis et al.

[ SUBSTITUTED SULFONYLACETAMIDO CEPHALOSPORINS [75] Inventors: Robert M. De Marinis, King of Prussia; John R. E. Hoover, Glenside, both of Pa.

[73] Assignee: SmithKline Corporation,

Philadelphia, Pa.

[22] Filed: Dec. 3, 1974 [21] Appl. No.: 529,168

Related US. Application Data [62] Division of Ser. No 249.858, May 3, 1972, Pat. No

[ Dec. 9, 1975 OTHER PUBLICATIONS Lewis et al., Antimicrobial Agents and Chemeoth erapy (1968) pp. 109-114 (1969).

Terao et al., Chemical Abstracts, V01. 79, 18, 739p (1973).

Sonia et al., Chemical Abstracts, Vol. 79, 105. 305n (1973).

Primary Examiner-R. Gallagher Assistant ExaminerDiana G. Rivers Attorney, Agent, or Firm-Stuart R. Suter; Alan D Lourie; William H. Edgerton [57] ABSTRACT Cephalosporin compounds having various substituted sulfonylacetamido groups at position 7 have been prepared and have antibacterial activity.

8 Claims, No Drawings SUBSTITUTED SULFONYLACETAMIDO CEPl-IALOSPORINS 2 triazolyl and the substituted derivatives thereof. Also preferred are compounds where X is methyl, ethyl, propyl. trifluoromethyl, phenyl, amino and dimethylamino.

This is a division of application Ser. No. 249,858 filed 5 The compounds of this invention are prepared by acon May 3, 1972, now U.S. Pat. No. 3,865,8l9. ylation of a 7-aminocephalosporan1c acld with the ap- This invention relates to cephalosporin compounds propriately substituted sulfonylacetic acid. The carwhich have antibacterial activity, in particular, comboxyl group is activated prior or during theacylatlon pounds having a substituted sulfonylacetamido substitreaction by one of the methods well known in the art. uent at position 7 of the cephem nucleus. l These include the mixed anhydride, acid hal de and ac The compounds of this invention are represented by tivated ester methods and the use of a coupling reagent the following structure. such as dicyclohexylcarbodiimide.

The alkyl and arylsulfonylacetic acids are prepared by oxidation of the appropriate substituted mercapto- 1 acetic acid with an oxidizing agent; for example, mex -T s thylmercaptoacetic acid is oxidized with m-chloropcrb benzoic acid to give methylsulfonylacetic acid. Alter- A natively the substituted mercaptoacetic acid can be 00M converted to an activated ester. such as the N-hydroxysuccinimide ester or the 2,4-dinitrophenyl ester, and

then oxidized to the activated sulfonylacetate. Aminowhere: sulfonylacetic acids (i.e., XSO,CH COOH where X is X is lower alkyl of C -C phenyl, unsubstituted or amino or dialkylamino) are prepared from commersubstituted with nitro, amino, dialkylamino, each cially available chlorosulfonylacetyl chloride by known alkyl having C,-C,, hydroxy, alkoxy (C,-C or methods (J. Amer. Chem. Soc, 8l,5655(1959); British 2 halogen; CF NR or mono or dialkylamino, each Pat. No. 1,067,965.

alkyl of C -C The 7-aminocephalosporins where A is CH S-Het are A is hydrogen, methyl, acetoxymethyl, pyridiniumprepared by known methods from 7-aminocephalospomethyl, CH,S-l-let, CH,SR, or CH,OR; ranic acid (7-ACA) and the appropriate heterocyclic Het is a 5 or 6-membered heterocyclic ring contain mercaptan compound. The compounds where A is ing one to four atoms selected firom the group conpyridiniummethyl are prepared by acylation of 7-ACA sisting of N, O and S, unsubstituted or substituted and then reaction with pyridine by known procedures. with from one to two groups selected from lower The compounds where A is hydrogen, methyl, alkylthialkyl, cycloalkyl or alkenyl, each having one to omethyl, or alkoxymethyl are prepared from materials four carbon atoms; lower alkoxy or alkoxyalkyl, known in the art, readily prepared by known methods each alkoxy or alkyl having one to four carbon or described herein. atoms; hydroxy; CF NHR; NR SR; or halogen; The compounds of this invention have broad-spec- R is hydrogen or lower alkyl (C -C and trum antibacterial activity with minimum inhibitory M is hydrogen, alkali metal cation, nontoxic ammo concentrations (MIC) ranging from 0.1 to 200 ug/ml. nium cation, or when A is pyridiniummethyl an ani- Table I shows MlCs for a variety of compounds within onic charge. the scope of this invention against various Gram-posi- Cephalosporins with a wide variety of acyl groups at tive and Gram-negative bacteria.

TABLE I g S. Strep. Kleb. Kleb. Pseu- Ente- Enteaur- S. Strep. l'ae- E. E. pneupnevdo. Salmo- Shirn. Serra. ro. eus aureus S. pyog. calis coli coii mo. mo. sp. nella gelia aerog. mare. cloaca Cmpd. HH SK vilc HH SK HH SK SK 111-1 ATCC HH ATCH ATCC PaSL N6. 127 23390 161111 203 34358 12140 3372 4200 1200 63 l2l76 112 131148 1311110 969 M16 (pig/ml] 54293 3.1 1.6 0.4 200 12.5 25 12.5 12.5 200 12.5 25 200 200 55848 1.6 1.6 0.2 12.5 25 3.1 6.3 200 3.1 12.5 200 200 57286 3.1 1.6 0.2 100 3.1 12.5 1.6 3.1 200 3.1 3.1 200 200 200 57359 1.6 0.8 0.2 so 50 100 12.5 6.3 200 12.5 :5 200 200 200 57360 0.11 0.4 0.1 25 25 100 25 12.5 200 25 25 200 200 200 59345 0.8 0.8 25 0.1 100 100 100 50 1110 200 50 50 200 200 200 59393 1.6 1.6 25 0.1 50 50 50 25 25 200 12.5 25 200 200 100 59454 25 25 200 0.4 200 200 200 200 200 200 200 200 200 200 200 59586 1.6 0.8 100 0.2 100 6.3 25 3.1 3.1 200 1.6 12.5 '200 200 200 59623 0.11 0.8 100 0.1 100 25 50 6.3 6.3 200 3.1 25 200 200 59641 0.4 0.4 100 0.1 25 3.1 12.5 1.6 1.6 200 L6 6.3 100 200 59758 3.1 1.6 50 0.1 200 6.3 12.5 6.3 6.3 200 3.1 0.1 200 200 591357 1.6 L6 25 0.2 100 25 50 6.3 6.3 200 3.1 25 200 200 'Scc Tuhle 2 for structures position 7 are known in the prior art including substi- TABLE 2 tuted sulfinylalkanoyl groups. The present invention relates to substituted sulfony]acetamidocephaiospo- Cumi Nu X A rms, having a doubled oxidized sulfur atom. 54293 methyl ucewxymuhyl Preferred compounds are those where A Is CH. .S-Het 5584s methyl S-methyl-l 3.441116111312612 I ylthiomethyl and in particular where Het is tetrazolyl, 1,3,4 572% methyl l melhylemml s yhhio thiadiazolyl, 1,3,4-oxadiazolyl, 1,2,4-triazolyl, l,2,3-

methyl TABLE Z-continued Compound No. X A

57359 phenyl aceloxymethyl 57360 phenyl 5-methyl-1,3,4-thiadiazol-2- ylthiomethyl 59345 methyl pyridiniummethyl 59393 amino acetoxymethyl 59454 methyl methyl 59586 methyl S-rnethyl- 1 .2.4-triazol3- ylthiomethyl 59623 ethyl acetoxymethyl 5964 l ethyl 5-methyll ,3,4-thiadiazo1-2- ylthiomethyl 597 58 methyl 4-methyll .2 .4-triazol- 3-ylthiomethyl 59857 methyl A-methyl-Soxol ,2,4-triazol 3-ylthiomethy1 These compounds are formulated and administered in the same manner as other cephalosporins with the dose depending on the subject and the infection being treated.

The following examples illustrate the invention but are not to be construed as limiting the scope thereof.

EXAMPLE 1 7-Methylsulfonylacetamidocephalosporanic acid Dicyclohexylcarbodiirnide (20.6 g, 0.1 mol) was added to a solution of methylmercaptoacetic acid 10.7 g, 0.10 mol) and N-hydroxysuccinimide (11.5 g, 0.1 mol) in dry tetrahydrofuran (100 ml). The mixture was stirred in an ice bath for one hour and then allowed to stand overnight. The precipitate was collected and the filtrate was evaporated to give the crude product which was recrystallized from carbon tetrachloride.

The above activated ester (4.06 g, 0.02 mol) was dis solved in chloroform (40 ml) and the solution was cooled to To this was added over a 15 minute period a solution of m-chloroperbenzoic acid (8.1 g, 0.04 mol) in ether (50 ml). The reaction is stirred one hour in an ice bath and three days at room temperature. The precipitated product was collected and was recrystallized from acetone to give the methylsulfonylacetate ester.

To a suspension of 7-ACA (1.64 g, 0.06 mol) in dry dimethylformarnide (20 ml) was added sufficient triethylamine to effect solution. The above ester (1.4 g, 0.06 mol) was added to the solution at room temperature with stirring. After 1.25 hours the reaction was poured into ice water and the aqueous mixture was acidified to pH: and extracted with ethyl acetate. The extracts were dried and concentrated to a volume of about 20 ml. An equal volume of ether was added followed by sodium 2-ethy1hexanoate and then more ether 100 ml.). The precipitate was collected, washed with ether, and dried to yield the title compound as its sodium salt. The free acid is obtained by acidification of an aqueous solution of the sodium salt.

EXAMPLE 2 7-Methylsulfonylacetamido-3-( l-methyltetrazol-S- ylthiomethyl )-3-cephem-4-carboxylic acid To a suspension of 7-amino-3-(l-methyltetrazol-S- ylthiomethyl )-3-cephem-4carboxylic acid (1.09 g, 3 mmol) in dimethylform-amide (25 ml) was added triethylamine until all the solid was dissolved. The activated methylsulfonylacetate ester from Example 1 (0.705 g. 3 mmol) was added to the solution in one portion. The reaction solution was stirred at room temper ature for hours and then was added dropwise to ether (200 ml). The gummy precipitate was stirred with filter aid and filtered. The filter cake was washed with ether, and then stirred with acetonitrile (50 m1) and filtered again. The filtrate was evaporated to a gummy residue which was dissolved in water (40 ml). The aqueous phase was extracted with ethyl acetate (40 m1), acidified to pH 2, and reextracted with ethyl acetate. The organic extracts of the acidic solution were washed with water, dried and concentrated to a volume of about 50 m1. Sodium 2-ethylhexanoate in isopropanol was added to precipitate the sodium salt of the title compound. The free acid is obtained from the sodium salt by standard methods.

EXAMPLE 3 7-Me thylsulfonylacetamido-3-( 2-methyl- 1 ,3 ,4- thiadiazol-S-ylthiomethyl)-3-cephem-4-carboxy1ic acid Triethylamine was added to a suspension of 7-amino- 3-( 2-methy1- 1 ,3 ,4-thiadiazol-5-ylthiomethyl )-3-cephem-4-carboxylic acid 1.72 g, 5 mmol) in dimethylformamide (25 m1) until a slight cloudiness remained. To this was added the activated sulfonyl ester from Example 1 (1.18 g, 5 mmol) and the reaction was stirred at room temperature for 2 hours. The solution was poured into ice water ml) and the resultant solution was extracted with ethyl acetate. The aqueous phase was acidified with 3N HCl to pH 2 and extracted with ethyl acetate. The combined extracts were washed with water, dried and concentrated to a volume of 50 ml. Sodium 2-ethylhexanoate was added and then ether (200 ml). The precipitated sodium salt was collected, washed with ether and dried. By standard methods, the free acid is obtained from the sodium salt.

EXAMPLE 4 7-Phenylsulfonylacetamido-3-(2-methy1- 1 ,3,4- thiadiazol-S-ylthiomethyl)-3-cephem-4-carboxy1ic acid When phenylmercaptoacetic acid was reacted with N-hydroxysuccinimide and then m-chloroperbenzoic acid according to the procedure in Example 1, N- hydroxysuccinimidyl phenylsulfonylacetate was obtained.

7-Amino-3-( 2-methyll .3 .4-thiadiazol-S-ylthiomethyl)-3-cephen1-4-carboxylic acid (1.38 g, 0.04 mol) was suspended in dimethylformamide (25 m1) and triethylamine was added until solution was effected. The above ester (1.1 g, 0.04 mol) was added and the reaction was stirred for 2 hours at room temperature. The solution was added dropwise to ether (200 ml). The suspension was filtered through filter aid and the filter cake was washed with ether. The product and filter aid were stirred with water (50 ml) for 20 minutes and refiltered. The aqueous filtrate was extracted with ethyl acetate (which was discarded) and then acidified to pH 1 with 3N l-lCl and reextracted with ethyl acetate. The extracts were washed with water, dried, and sodium 2- ethylhexanoate was added. The sodium salt was collected and dried. An aqueous solution of the sodium salt is adjusted to pH 2 and the free acid is collected.

EXAMPLE 5 7-Phenylsulfonylacetamidocephalosporanic acid When the activated phenylsulfonylacetate of Exampie 4 was reacted with 7-ACA according to the same procedure as Example 4, the title compound was obtained.

EXAMPLE 6 7 -Trifluoromethylsulfonylacetamido-3 2-methyl l ,3,4-thiadiazol-5-ylthiomethyl )-3-cephem-4-carboxylic acid Trifluoromethylmethylsulfone (1.48 g, 0.01 mol) [W. E Truce, et al., J. Amer. Chem. Soc, 74, 3594 1952)] was dissolved in dry ether (50 ml) and the solution was cooled sitrring. -40. A 2 molar solution of n-butyl lithium (7.5 ml, 0.015 mol) was added; the reaction was warmed to ca. stirred for 30 minutes, and then poured over crushed CO with stirring. When the mixture reached room temperature it was shaken with water (75 ml); the aqueous phase was separated, acidified to pH 1.5, and extracted with ether. The extracts were dried and evaporated to give an oil which crystallized on standing. The trifluoromethylsulfonylacetic acid was recrystallized from carbon tetrachloride.

Using the procedure in Example 1 trifluoromethylsulfonylacetic acid is esterified with N-hydroxysuccinimide and then is reacted with 7-amino-3-(2-methyl- 1,3 ,4-thiadiazol-5-ylthiomethyl )-3-cephem-4-carboxylic acid using the procedure of Example 3 to give the title compound.

EXAMPLE 7 7-Trifluoromethylsulfonylacetamidocephalosporanic acid The reaction of the activated ester of trifluoromethylsulfonylacetic acid from Example 6 with 7-ACA according to the procedure of Example l gives the title compound.

EXAMPLE 8 7-Aminosulfonylacetamidocephalosporanic acid Dicyclohexylcarbodiimide (l g, 5 mmol) was added to a solution of 7-ACA t-butyl ester (1.65 g, 5 mmol) and aminosulfonylacetic acid (0.7 g, 5 mmol) (British Patent No. 1,067,965) in dry tetrahydrofuran (15 ml). The solution was stirred overnight at room temperature, filtered and the precipitate was washed with tetrahydrofuran. The filtrate and washings were combined and evaporated to a gum. The gum was reacted with trifluoroacetic acid (5 ml) for 15 minutes at room temperature and then the reaction solution was added dropwise to ether (150 ml). The precipitate was filtered, washed with ether, then stirred with ethyl acetate and filtered. The filtrate was treated with sodium 2- ethylhexanoate in isopropanol. The precipitated sodium salt was collected, washed with ether and acetonitrile and dried. The sodium salt is converted to the free acid by standard methods.

EXAMPLE 9 7-Aminosulfonylacetamido-3-(Z-methyl-1,3,4- thiadiazol-S-ylthiomethyl )-3-cephem-4-carboxylic acid Using the procedure of Example 3, 7-amino-3-(2- methyl- 1 ,3 ,4-thiadiazol-5 -ylthiomethyl )-3-cephem-4- carboxylic acid is reacted with N-hydroxysuccinimidyl aminosulfonylacetate (prepared in a similar manner as set forth in Example 1 from aminosulfonylacetic acid and N-hydroxysuccinimide) to give the desired product.

EXAMPLE 10 When an equivalent amount of a 7-amino-3- heterocyclicthiomethyl-3-cephem-4-carboxylic acid listed below is substituted in Example 2 for 7-amino-3- 1-methyltetrazol-S-ylthiomethyl )-3-cephem-4-carboxylic acid, the appropriate 7-methylsulfonylacetamido-3-heterocyclicthiomethyl-3-cephem-4- carboxylic acid is obtained.

7-Amino-3-( l ,3,4-thiadiazol-Z-ylthiomethyl )-3-cephem-4-carboxylic acid 7-Amino-3-(S-trifluoromethyl-1,3.4-thiadiazol-2-ylthiomethyl)-3-cephem-4-carboxylic acid 7-Amino-3-(5-ethyll ,3,4-thiadiazol-2-ylthiomethyl 3-cephem-4-carboxylic acid 7-Amino-3-( S-n-butyl-l ,3 ,4-thiadiazol2-ylthiomethyl)-3-cephem-4-carboxylic acid 7-Arnino-3-( S-dimethylaminol ,3,4-thiadiazol-2-ylthiomethyl )-3-cephem-4-carboxylic acid 7-Amino-3-( S-mercaptol ,3,4-thiadiazol-3-ylthiomethyl)-cephe m-4-carboxylic acid 7-Amino- 3-(3-methylthio-1,2,4-thiadiazol- S-ylthiomethyl)-3-cephem-4-carboxylic acid 7-Amino- 3-( 3-methyl-l ,2,4-thiadiazol-5-ylthiomethyl)-3-cephem-4-carboxylic acid 7-Amino-3-(tetrazol-iylthiomethyl )-3-cephem-4-c arboxylic acid 7-Amino-3-( l,2,4-triazol-3-ylthiomethyl)-3-cephem-4- carboxylic acid 7-Amino-3-ylthiomethyl )3-cephem-4-carboxylic acid 7-Amino-3-(4-methyl- 1 ,2,4-triazo|-3-ylthiomethyl)- 3- cephem-4-carboxylic acid 7-Amino-3-(4,5-dimethyl-1 ,2.4-triazol3-ylthiomethyl )-3-cephem-4-carboxylic acid 7-Amino-3-(2,5-dimethyl-1,2,4triazol-3-ylthiomethyl)-3-cephem-4-carboxylic acid 7-Amino- 3-( 4-methyl-5-trifluoromethyll ,2 ,4-triazol- 3-ylthiomethyl)-3-cephem-4carboxylic acid 7-Amino- 3-( S-ethyl-l ,2,4-triazol- 3-ylthiomethyl )-3- cephem-4-carboxylic acid 7-Amino- 3-( 4-ethyl- 1 ,2,4triazol-3-ylthiomethyl )-3- cephem-4-carboxylic acid 7-Amino-3-( 2-methyll ,2,4-triazol-3-ylthiomethyl)-3- cephem4-carboxylic acid 7-Amino- 3-( 2-ethyll ,2,4-triazol-3-yl thiomethyl )-3- cephem-.4-carboxylic acid 7-Amino-3-(4-allyll ,2,4-triazol-3-ylthiomethyl )-3- cephem-4-carboxylic acid 7-Amino-3-(5-methoxymethyl-1,2,4-triazoL3-ylthiomethyl)-3-cephem-4-carboxylic acid 7-Amino-3-(5-cyclopropyl-1 ,2,4-triazol-3-ylthiomethyl)-3-cephem-4-carboxylic acid 7-Amino-3-( 5-bromo- 1 ,2,4-triazol-3-ylthiomethyl 3- cephem-4-carboxylic acid 7-Amino- 3-( 5-hydroxyl ,2,4-triazol-3 -ylthiomethy1)- 3-cephem-4-carboxylic acid 7-Amino- 3-( 5-hydroxy-4-methyl-1 ,2,4-triazol-3-yl-thiomethyl)-3-cephem-4-carboxylic acid 7-Amino- 3-(5-hydroxy-4-ethyll ,2,4-triazol-3-yl-thiomethyl)-3-cephem-4-carboxylic acid 7-Amino-3-( 1,2,3-triazol-S-ylthiomethyl )-3-cephem-4- carboxylic acid 7-Amino-3-( l ,3,4-oxadiazol-2-ylthiomethyl )-3-cephem-4-carboxylic acid 7-Arnino- 3-( S-methyll ,3 ,4-oxadiazol-2-ylthiomethyl 3-cephem-4-carboxylic acid 7-Amino-3 4-pyrimidylthiomethyl)-3-cephem-4-carboxylic acid 7-Amino-3 2-pyrazinylthiomethyl )-3-cephem-4-carboxylic acid 7-Amino- 3-( B-pyridylthiomethyl )-3-cephem-4-carboxylic acid 7-Amino-3-( 4-pyridylthiomethyl 3-cephem-4carboxylic acid EXAMPLE 1 l The reaction of 7-amino-3-( l-methyltetrazol-S-ylthiomethyl )-3-cephem-4-carboxylic acid or a 7-amino- 3-heterocyclicthiomethyl-3-cephem-4-carboxylic acid enumerated in Example 10 with activated phenylsulfonylacetate ester according to the procedure of Example 4 gives the appropriate 7-phenylsulfonylacetamido-3- heterocyclicthiomethyl-3-cephem-4-carboxylic acid.

EXAMPLE 12 When the procedure of Example 6 is followed using 7-amino-3-( l-methyltetrazol-S-ylthiomethyl)-3-cephem-4-carboxylic acid or a 7-amino-3-heterocyclicthiomethylcephalosporin listed in Example 10, the corre sponding 7-trifluoromethylsulfonylacetamido3- heterocyclicthiomethyl-3-cephem-4-carboxylic acid is obtained.

EXAMPLE l3 Substitution of 7-amino-3-( l-methyltetrazol-S-ylthiomethyl)-3 cephem-4-carboxoylic acid or a cephalosporin compound listed in Example 10 in the procedure of Example 9 gives the appropriate 7-aminosulfonylacetamido-3-heterocyclicthiomethyl-3-cephem-4- carboxylic acid product.

EXAM PLE l4 7-Methylsulfonylacetamido-3-( l-pyridiniummethyl)-3- cephem-4-carboxoylic acid To a solution of 7-methylsulfonylacetamidocephalos poranic acid sodium salt 1.75 g, 4.0 mmol) in water (2 ml) was added potassium thiocyanate (7.87 g, 0.08l mol), water (2 ml) and pyridine (0.44 ml). The reaction was heated at 6570 for 7 hours and then was cooled. The solution was diluted with water (75 ml) and the aqueous solution was chromatographed on a column of cross-linked polystyrene polymer (125 g of Amberlite XAD-2). The inorganic salts were eluted with water and then the product was eluted with 95% ethanol. The ethanol fractions were evaporated to give the crude product which was dissolved in water filtered and lyophilized.

EXAMPLE 15 When trifluoromethylsulfonylacetamidocephalosporanic acid, aminosulfonylacetamidocephalosporanic acid, or phenylsulfonylacetamidocephalosporanic acid is substituted for methylsulfonylacetamidocephalosporanic acid in Example 14 the corresponding 3-(1- pyridiniummethyl)cephalospoiin is obtained.

EXAMPLE 16 7-( N,N-Dimethylaminosulfonylacetamido )-3-( 2-meth yll ,3,4-thiadiazol-5-yl )-3-cephem-4-carboxylic acid N,N-Dimethylaminosulfonylacetic acid is prepared in an analogous method as aminosulfonylacetic acid and then is reacted with 7-amino-3-(2-methyl-l,3,4- thiadiazol-S-yl-thiomethyl )-3-cephem-4-carboxylic acid according to the procedure of Example 9 to give the title compound.

EXAMPLE 17 When 7-amino-3-( 1-methyltetrazol-S-ylthiomethyl 3cephem-4-carboxylic acid or a 7-amino-3-heterocyclic cephalosporin enumerated in Example 10 is reacted with N,N-dimethylaminosulfonylacetic acid by the procedure of Example 16 the corresponding 7- (N,N-dimethylaminosulfonylacetamido)-3-heterocyclicthiomethyl-3-cephem-4-carboxylic acid is obtained.

EXAMPLE IS The reaction of N,N-dimethylaminosulfonylacetic acid with 7-ACA t-butyl ester according to the procedure of Example 8 gives 7-(N,N-dimethylaminosu1- fonylacetamido)cephalosporanic acid.

When the above product is reacted with pyridine using the procedure of Example 14, 7-(N,N-dimethylaminosulfonylacetamido)-3-( lpyridiniummethyl)- 3-cephem-4-carboxylic acid is obtained.

EXAMPLE l9 7-Methylsulfonylacetamido-3-methyl-3-cephem-4-c arboxylic acid 7-Amino-3-methyl-3-cephem-4-carboxylic acid (7- ADCA) (2.l4 g, 0.01 mol) was suspended in dry dimethylformamide (30 ml) and l,5-dia.zobicyclo[4.3.0]non-5-ene was added until only a slight cloudiness remained. The activated ester from Example l (2.35 g, 0.01 mol) was added and the reaction was stirred for 2.5 hours at room temperature. The solution was filtered into ether (300 ml) and then the filtrate was filtered through filter aid. The filter cake was stirred with water (50 ml) and refiltered. The filtrate was acidified to pH 1.5 with 3N HCl and extracted with ethyl acetate. The organic extracts were washed with water, dried, and concentrated to ca. 50 ml. Sodium 2- ethylhexanoate was added and the precipitated sodium salt was collected, washed with ethyl acetate and dried. The free acid is obtained from the sodium salt by standard methods.

EXAMPLE 20 When the activated esters of phenylsulfonylacetic acid, trifluoromethylsulfonylacetic acid, aminosulfonylacetic acid, or N,N-dimethylaminosulfonylacetic acid are reacted with 7-ADCA according to the procedure in Example l9 the corresponding 7-sulfonylacetamido-3-methyl-3-cephem-4-carboxylic acid is obtained.

EXAMPLE 21 7-Ethylsulfonylacetamidocephalosporanic acid N-hydroxysuccinimidyl ethylsulfonylacetate was prepared from ethylmercaptoacetic acid according to the procedure of Example 1. To a suspension of 7-ACA (2.18 g, 8 mmol) in dry dimethylformamide (50 ml) was added triethylamine until solution was effected and then the activated ester (1.99 g, 8 mmol) was added in one portion. The resultant reaction mixture was stirred 1.5 hours at room temperature and then poured with stirring into ether (400 ml) which caused a gummy residue to precipitate. The mixture was filtered through Celite and the filter cake was stirred with water ml) until the residue was dissolved. The aqueous solution was filtered, washed with ethyl acetate, covered 9 with fresh ethyl acetate and acidified with 3N l-lCl to pH 2. The acidic solution was extracted with ethyl acetate. The combined extracts were washed with water, dried, and evaporated to give the title compound.

EXAMPLE 22 7-Ethylsulfonylacetamido-3-( 2-methyll ,3,5- thiadiazol-S-yl-thiomethyl )-3-cephem-4-carboxylic acid Using the activated 7-ethylsulfonylacetate ester (1.99 g, 8 mmol) and 7-amino-3-(2-methyl-l,3,5- thiadiazol-S-ylthiomethyl )-3-cephem-4-carboxylic acid (2.75 g, 8 mmol) in the procedure of Example 21, the title compound was prepared.

EXAMPLE 23 When 7-amino-3-( l-methyltetrazol-S-ylthiomethyl 3-cephem-4-carboxylic acid or any of the compounds enumerated in Example 10 are substituted for 7-ACA in the procedure of Example 2] the corresponding 7- ethylsulfonylacetamido-3-heterocyclicthiomethyl-3- cephem-4-carboxylic acid is obtained.

Similarly, 7-ethylsulfonylacetamido-3-methyl-3- cephem-4-carboxylic acid is obtained by acylating 7- ADCA using the procedure of Example 2i.

EXAMPLE 24 Propylmercaptoacetic acid is converted into N- hydroxysuccinimidyl propylsulfonylacetate in the same manner as the methyl analog in Example I. This ester is reacted with 7-ACA, 7-ADCA, 7-amino-3-(2-methyll ,3 ,4-thiadiazol-5-ylthiomethyl )-3-cephem-4-carboxylic acid, 7-amino-3-( l-methyl-tetrazol-5-ylthiomethyl)- 3-cephem-4-carboxylic acid, or any of the compounds enumerated in Example according to the procedure of Example 2| to give the appropriate 7-propylsulfonylacetamidocephalosporin.

EXAMPLE 25 Acylation of 7-amino-3-methylthiomethyl-3-cephem-4-carboxylic acid (Belgian Pat. No. 743,754) with the activated esters of methylsulfonylacetic acid, ethylsulfonylacetic acid, or propylsulfonylacetic acid ac cording to the procedure of Example 1 gives the corresponding 7-alkylsulfonylacetamido-3-methylthiomethyl-3-cephem-4-carboxylic acid. Using the procedure of Example 8 the above cephem nucleus is acylated with dimethylaminosulfonylacetic acid to give 7-dimethylaminosulfonylacetamido-3-methylthiomethyl-3- cephem-4-carboxylic acid.

EXAMPLE 26 Using the procedure of Example 1, 7-amino-3- methoxymethyl-3-cephem-4-carboxylic acid [1. Med. Chem, 14, ll3(197l)] is acylated with the activated ester of methylsulfonylacetic acid, ethylsulfonylacetic acid, or propylsulfonylacetic acid to give the corresponding 7-alkylsulfonylacetamido-3-methoxymethyl- 3-cephem-4-carboxylic acid. The same cephem nucleus is acylated with dimethylaminosulfonylacetic acid by the procedure of Example 8 to give 7-dimethylaminosulfonylacetamido-3-methoxymethyl-3- cephem-4-carboxylic acid.

EXAMPLE 27 3-Formyl-7-(2-thienylacetamido)-2-cephem-4-carboxylic acid 7-(2-Thienylacetamido)cephalosporanic acid (45 g, 0.11 mol) was dissolved in dry pyridine (I35 ml) by warming. This solution was cooled in an ice bath and acetic anhydride (13.5 ml) was added with stirring. After stirring 0.5 hour the reaction was stored in a refrigerator 18 hours during which time the mixture solidified. Ether was added to the mixture and the solid pyridine salt was collected and washed with ether. The salt was suspended in ethyl acetate (400 ml) with stirring and was collected again. The salt was then dissolved in a mixture of water (300 ml), ethyl acetate (400 ml) and 2N I-lCl (54 ml); the aqueous phase was saturated with NaCl and then was separated. The organic phase was washed with saturated NaCl solution, filtered through coarse filter paper three times, and evaporated to yield the A -isomer (32.4 g).

The A -isomer (32.4 g) was suspended in a mixture of acetone (60 ml) and water (300 ml) and 5% Na,CO solution was added until pH 10.5 was reached. The so lution was allowed to stand two days during which time additional 5% Na,CO was added several times to maintain the solution at pH [0.5. The reaction was cooled in ice, layered with ethyl acetate and acidified with 6N H to pH 1.8. The aqueous phase was saturated with NaCl and separated. The organic phase was washed with saturated NaCl solution, filtered through coarse filter paper and evaporated to give the product, the N-B-hydroxymethyl compound (17.4 g).

A solution of the above product (17.4 g, 0.049 mol) in acetone (850 ml) was cooled to -l0 and then Jones reagent [chromic trioxide (26.72 g) dissolved in concentrated H 80, (23 ml) and then diluted to I00 ml with water] was added dropwise until the brown color persisted. Excess Jones reagent was decomposed with isopropanol, the green reaction mixture was filtered and the green pricipitate was washed with acetone. The combined filtrate and washings were diluted with water (200 ml) and ethyl acetate (400 ml) and then saturated with NaCl. The organic phase was separated, washed with saturated NaCl solution and dried over MgSO,. Evaporation of the solvent gave the crude product which was triturated with methanol to give the product as its methanoate (8.4 g). This product was dissolved in ethyl acetate (2000 ml) and then evaporated in vac no to give the title compound (7.8 g).

EXAMPLE 28 Benzhydryl 3-formyl-7-( Z-thienylacetamido)-2-cephem-4-carboxylate to half volume. The product was precipitated by the addition of hexane.

EXAMPLE 29 Benzhydryl-7-( 2-thienylacetamido)-3-cephem-4-carboxylate A mixture of the benzhydryl ester from Example 28 (2.5 g, 4.79 mmol), tris-(triphenylphosphine)chlororhodium (3.5 g. 4.79 mmol) and dry toluene (75 ml) was refluxed under nitrogen for 5.5 hours. The reaction mixture was cooled. the rhodium complex was re moved by filtration and the filtrate was poured slowly into petroleum ether (250 ml) with stirring. The precipitated solid, which was a mixture of rhodium complex and product, was collected. The product was purified by chromatography on a silica gel column (250 g) using 9905 chloroformzethyl acetate as eluent. Fractions of 4.5 ml were collected; fractions 1-47 discarded, 48-66 contained bis-(triphenylphosphinc)carbonylchlororhodium, and 67l98 contained the product. NMR data indicated the product after chromatography to be the A isomer instead of the A isomer. A decoupling experiment showed the Z-methylene group at 3.3 ppm. to be coupled with the 3-proton at 6.6 ppm.

EXAMPLE 30 Benzhydryl 7amino-3-cephem-4-carboxylate Benzhydryl 7-( 2-thienylacetamido)-3-cephcm-4-carboxylate (2.45 g. 5 mmol) was dissolved in calcium hydride dried benzene (130 ml) containing dry pyridine (0.59 g, 7.4 mmol). The solution was maintained at and PCl l .54 g. 7.4 mmol.) was added with stirring. The reaction mixture was stirred under nitrogen at 0 for three hours and then the solvent was removed in vacuo. Anhydrous methanol (260 ml) was added to the residue and the resultant mixture was allowed to stand two hours. The methanol was removed in vacuo and the residue was treated with 20% aqueous tetrahydrofuran. After 15 minutes the tetrahydrofuran was removed in vacuo and the aqueous residue was adjusted to pH 7.5 and extracted with ethyl acetate. The extracts were washed with water, dried over MgSO and evaporated to give the solid product which was triturated with chloroform-petroleum ether and dried under high vacuum.

EXAMPLE 3 l 7-Methylsulfonylacetamido-3-cephem-4-carboxylic acid A solution of benzhydryl 7-amino-3-cephem-4-carboxylate (0.78 g, 2 mmol), methylsulfonylacetic acid (0.27 g, 2 mmol). and dicyclohexylcarbodiimide (0.4 g, 2 mmol) in dry tetrahydrofuran (15 ml) is stirred at room temperature overnight. The precipitate is collected and washed with tetrahydrofuran and the combined filtrate and washings are evaporated in vacuo. The residue is treated with a cold solution of trifluoroacetic acid (10 ml) and anisole (0.5 g) for minutes and then concentrated in vacuo. The residue is dissolved in ethyl acetate and treated with 5% NaHCO The aqueous phase is adjusted to pH 2 and the precipitated product is collected and dried.

EXAMPLE 32 When ethylsulfonylacetic acid, propylsulfonylacetic acid, trifluorornethylsulfonylacetic acid, phenylsulfonylacetic acid, aminosulfonylacetic acid, or N,N- dimethylaminosulfonylacetic acid is substituted for methylsulfonylacetic acid in the procedure of Example 3 l. The corresponding lsulfonylacetamido-3-cephem-4- carboxylic acid is obtained.

EXAMPLE 33 An injectable pharmaceutical composition is formed by adding sterile water or sterile saline solution (2 ml) to 500 mg of sodium 7-methylsulfonylacetamido-3-( lmethyltetrazol-S-ylthiomethyl)-3-cephem-4-carboxylate.

Pharmaceutical compositions of the other antibacterial compounds disclosed above may be formulated in a similar manner.

it is recognized that when the substituent on the heterocyclic group is hydroxy or mercapto that it is possible for the substituent to exist in another tautomeric form, i.e. the oxo or thiono form. The compounds may exist exclusively as one of the two tautomers or may be in equilibrium between the two; however, these are all included within the scope of this invention.

What we claim is:

l. A compound of the structure:

COOM

where:

X is lower alkyl of C -C A is hydrogen, methyl, acetoxymethyl, or pyridiniummethyl; and

M is hydrogen, alkali, metal cation, nontoxic ammonium cation, or when A is pyridiniurnmethyl an anionic charge.

2. A compound as claimed in claim 1 where X is methyl, ethyl, or propyl.

3. A compound as claimed in claim 2 being the compound 7-methylsulfonylacetamidocephalosporanic acid.

4. A compound as claimed in claim 2 being the compound 7-ethylsulfonylacetamidocephalosporanic acid.

5. A compound as claimed in claim 2 being the compound 7-methylsulfonylacetamido-3-cphem-4-carboxylic acid.

6. A compound as claimed in claim 2 being the compound 7-ethylsulfonylacetamido-3-cephem-4-carboxylic acid.

7. A compound as claimed in claim 2 being the compound 7-methylsulfonylacetamido-3-methyl-3-cephem-4-carboxylic acid.

8. A compound as claimed in claim 2 being the compound 7-methylsulfonylacetamido-3-( l-pyridiniummethyl)-3-cephem-4-carboxylic acid.

o a: v t

UNITED STATES PATENT OFFICE CERTIFICATE OF CORRECTION Patent No. 3,925,373 Dated December 9, 1975 Inventor(s) Robert M. DeMarinis and John R. E. Hoover It is certified that error appears in the above-identified patent and that said Letters Patent are hereby corrected as shown below:

Column 3, line 49, "pHz" should read pH 2 Column 5, line 12, "sitrring." should read to Column 6, line 32, "7-Amino-3-ylthi.omethyl)-3-cephem-4 carboxylic acid" should read 7-Amino-3-(5-methyl-l,2,4- triazol-B-ylthiomethyl) -3-cephem +-carboxy1ic acid Signed and Scaled this eighth Day of June1976 [SEAL] Aum:

RUTH C. MASON C. MARSHALL DANN Arresting Officer Commissioner uj'larem: and Trademark: 

1. A COMPOUND OF THE STRUCTURE:
 2. A compound as claimed in claim 1 where X is methyl, ethyl, or propyl.
 3. A compound as claimed in claim 2 being the compound 7-methylsulfonylacetamidocephalosporanic acid.
 4. A compound as claimed in claim 2 being the compound 7-ethylsulfonylacetamidocephalosporanic acid.
 5. A compound as claimed in claim 2 being the compound 7-methylsulfonylacetamido-3-cephem-4-carboxylic acid.
 6. A compound as claimed in claim 2 being the compound 7-ethylsulfonylacetamido-3-cephem-4-carboxylic acid.
 7. A compound as claimed in claim 2 being the compound 7-methylsulfonylacetamido-3-methyl-3-cephem-4-carboxylic acid.
 8. A compound as claimed in claim 2 being the compound 7-methylsulfonylacetamido-3-(1-pyridiniummethyl)-3-cephem-4-carboxylic acid. 